Description

Buy Methylene Blue EU | High Purity Research Chemical MBS Certified

Welcome to eupeptideshop.org, your premium source for high-purity research peptides and research chemicals in Europe. If you are looking to buy Methylene Blue EU for your laboratory investigations, you have found the right supplier. Our Methylene Blue (CAS: 61-73-4) is meticulously produced to meet the rigorous demands of scientific research, ensuring you receive a consistent and reliable product for your studies.

As a trusted research peptides EU supplier, we understand the critical importance of purity and accuracy. Our Methylene Blue is not for human or veterinary use; it is strictly intended for in vitro research and laboratory applications. Researchers across Germany, France, the Netherlands, and Spain consistently choose us for their laboratory peptides Netherlands and peptide supplier Spain needs due to our unwavering commitment to quality and compliance.

Product Overview: Methylene Blue for Research

Methylene Blue, also known as Basic Blue 9 or Methylthioninium chloride, is a heterocyclic aromatic compound with a wide range of applications in biological and chemical research. Its unique redox properties and ability to interact with nucleic acids make it an indispensable tool in the modern laboratory. Whether you are conducting assays in peptide synthesis Europe or developing novel diagnostic platforms, our high-purity Methylene Blue provides the foundation for reproducible and accurate results .

Key Technical Specifications

Researchers seeking to buy Methylene Blue require precise technical data to ensure compatibility with their experimental protocols. Below are the verified specifications for our product:

• Chemical Name: Methylthioninium chloride

• Synonyms: Basic Blue 9, CI-52015

• CAS Number: 61-73-4

• Molecular Formula: C16H18ClN3S

• Molecular Weight: 319.85 g/mol

• Purity: ≥98% (HPLC)

• Purity Note: Our product exceeds standard dye-grade purity (>70%) by utilizing advanced HPLC purification methods to remove related impurities, ensuring suitability for sensitive assays .

• Appearance: Dark green crystalline powder (solution yields a characteristic blue color).

Solubility and Stability Profiles

Proper handling and storage are essential for maintaining the integrity of this research chemical EU compliant product.

• Solubility Profiles:

  • Water: Soluble up to 30 mg/mL (with warming) .

  • DMSO: Soluble up to 62 mg/mL (with warming) .

  • Ethanol: Slightly soluble (up to 10 mg/mL with warming) .

• Recommended Storage Conditions:

  • Powder Form: Store lyophilized at -20°C. Under these conditions, the product is stable for up to 36 months. It can also be stored at 4°C for up to 2 years .

  • In Solution: Due to stability concerns in liquid form, it is imperative to prepare stock solutions fresh or aliquot and store at -20°C for a maximum of 1-6 months. Avoid repeated freeze-thaw cycles .

• Stability Data: As a photosensitizer, Methylene Blue should be protected from prolonged exposure to intense light to prevent degradation .

Known Research Applications

Methylene Blue is a versatile tool with a robust history in scientific literature. Our clients, ranging from peptide review blogs to advanced laboratories, utilize this compound for a variety of studies:

1. Electrochemical Biosensing: Methylene Blue is widely employed as a redox label in electrochemical sensors. It acts as an electroactive tracer, facilitating electron transfer for the detection of biomolecules like DNA and peptides . Recent advancements have utilized it as a signal tracer in nucleic acid-based lateral flow assays (NALFA) for colorimetric and electrochemical readouts .

2. Nucleic Acid Intercalation: MB is known for its ability to intercalate into double-stranded DNA. This property is exploited in studies of DNA damage, cross-linking, and sequence-selective DNA recognition, often in conjunction with peptide conjugates .

3. Nanozyme Activity: Recent research highlights the catalase-like activity of Methylene Blue when grafted onto peptide supports, enabling the ultrasensitive detection of small molecules like hydrogen peroxide .

4. Diagnostic Assays: Historically, it has been used as a staining dye in chromoendoscopy to identify dysplasia in gastrointestinal mucosa .

5. Inhibition Studies: MB is utilized in biochemical assays to study protein aggregation, such as the inhibition of tau filament formation, with an IC50 of 1.9μM .

Proper Handling and Reconstitution Guidelines

For researchers in Germany searching for high purity peptides Germany or those in France sourcing peptides for research France, correct protocol is non-negotiable.

• Safety Considerations: This product is for research use only. Handle using appropriate personal protective equipment (PPE), including gloves and safety glasses. Work in a well-ventilated area or fume hood.

• Handling Protocols: Minimize exposure to air and light. Keep the container tightly sealed in a dry, cool place. Avoid inhalation and contact with skin or eyes.

• Reconstitution Guidelines:

  1. Equilibrate: Allow the vial to reach room temperature before opening to prevent moisture condensation.

  2. Prepare Solvent: Use sterile water, PBS, or DMSO. Warming the solvent to room temperature or slightly above may aid dissolution .

  3. Calculate Volume: Use the formula: Volume (mL) = (Desired Concentration in mg/mL) / (Mass of Compound in mg). For example, to make a 10 mg/mL stock, add 1 mL of solvent to 10 mg of Methylene Blue.

  4. Aliquot: Divide the solution into single-use aliquots in sterile vials to avoid repeated freeze/thaw cycles.

  5. Storage: Store aliquots at -20°C. Use within one month to maintain potency .

Why Purchase from eupeptideshop.org?

When you decide to buy Methylene Blue, you are investing in the accuracy of your research. We are more than just an ecommerce store; we are a partner in the scientific community.

• Uncompromising Purity: Our >98% HPLC purity guarantee is backed by Certificate of Analysis (COA) data, ensuring that you are not working with low-grade dye containing impurities that could skew your results .

• Compliance: All our products, including this one, are classified as research chemicals EU compliant, suitable for non-human, in vitro applications.

• European Logistics: Based in the EU, we offer fast and reliable shipping across the continent. Whether you need buy peptides EU delivery to a lab in Berlin, a university in Lyon, or a research institute in Madrid, we ensure your order arrives safely and discreetly.

• Expert Support: Our team understands the technical nuances of peptide chemistry. If you have questions about solubility or applications, we are here to help.

Frequently Asked Questions (FAQ)

Here are some of the most common questions we receive from researchers looking to buy Methylene Blue and other research peptides EU products.

1. What is the exact purity of Methylene Blue sold at eupeptideshop.org?
Our Methylene Blue is tested via High-Performance Liquid Chromatography (HPLC) to guarantee a purity of ≥98%. This is significantly higher than standard industrial dyes, which often have purities below 70% . This high purity is essential for consistent and reproducible laboratory peptides Netherlands and across Europe.

2. How should I store Methylene Blue powder upon arrival?
The lyophilized powder should be stored desiccated at -20°C for long-term stability (up to 3 years). For short-term use, storage at 4°C in a dark, dry place is acceptable. Always keep the vial tightly sealed to prevent moisture absorption .

3. Can I use this Methylene Blue for human consumption or injection?
Absolutely not. This product is explicitly labeled for research use only. It is not a pharmaceutical grade, food grade, or a medicine. It is intended solely for in vitro research and laboratory purposes by qualified professionals. Please adhere to all local regulations regarding research chemicals EU compliant usage.

4. What is the solubility of Methylene Blue in DMSO?
Methylene Blue is soluble in DMSO up to approximately 62 mg/mL with gentle warming. It is also highly soluble in water (up to 62 mg/mL) and slightly soluble in ethanol (10 mg/mL) .

5. What are the main applications for Methylene Blue in peptide research?
Methylene Blue is often conjugated to peptides to study redox reactions, create electrochemical sensors for detecting protease activity (like MMP9), or investigate DNA-peptide interactions. It serves as an excellent electrochemical label due to its reversible redox activity . It is a staple for those conducting advanced peptide synthesis Europe projects.

6. Do you ship to Germany, France, and Spain?
Yes. We specialize in fast, tracked shipping across the entire European Union. Whether you are looking for high purity peptides Germany, peptides for research France, or a reliable peptide supplier Spain, our logistics network ensures your order arrives promptly and securely.

7. How do I prepare a stock solution for a cell culture experiment?
First, calculate the required mass. Dissolve the powder in a suitable solvent like sterile water or DMSO. It is crucial to filter-sterilize the final solution using a 0.22 µm filter before adding to cell cultures to maintain sterility. Always consult literature for acceptable solvent concentrations for your specific cell line.

8. Is Methylene Blue stable in solution?
Methylene Blue in solution is less stable than in lyophilized powder form. It is generally recommended to use solutions within one month when stored at -20°C. For aqueous solutions, degradation can occur faster at room temperature or under light exposure .

9. What safety data is available for this product?
Safety Data Sheets (SDS) and Certificates of Analysis (COA) are available upon request or for download on the product page. We encourage all researchers to review this information thoroughly before use to understand proper handling and safety protocols.

10. Why does my Methylene Blue solution appear purple instead of blue?
This can sometimes occur due to the concentration, the solvent used, or the pH of the solution. Aggregation of MB molecules at high concentrations can also shift the absorption spectra. If you are concerned about product integrity, please contact our support team with your lot number.

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